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A single magnetic field exposure system for sequential investigation of real time and downstream cellular responses
Author(s) -
Rao Raj R.,
Kisaalita William S.
Publication year - 2004
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.10151
Subject(s) - inverted microscope , bioelectromagnetics , amplifier , blinding , electromagnetic field , microscope , physics , optics , electromagnetic compatibility , magnetic field , extremely low frequency , acoustics , materials science , biological system , electrical engineering , optoelectronics , engineering , biology , bioinformatics , cmos , quantum mechanics , clinical trial
To be able to correlate real time membrane potential or ion flux changes with further downstream gene transcription responses due to extremely low frequency (ELF) electromagnetic field (EMF) exposure, we devised an experimental system consisting of a pair of symmetric circular coils. This system can be used on an inverted microscope stage (real time signaling) as well as inside controlled environment incubators (gene transcription end points). The system includes a unique, custom made switch box for blinding the experimental staff and a power amplifier. We report herein the design and characterization of the system with respect to parameters considered important in in vitro ELF–EMF exposure studies, including linear magnetic field distribution, compensation for microscope objective lens interference, heating effects of the coils, and harmonic content of the signals. Bioelectromagnetics 25:27–32, 2004. © 2003 Wiley‐Liss, Inc.