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Millimeter wave exposure reverses TPA suppression of gap junction intercellular communication in HaCaT human keratinocytes
Author(s) -
Chen Q.,
Zeng Q.L.,
Lu D.Q.,
Chiang H.
Publication year - 2004
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.10140
Subject(s) - hacat , photobleaching , gap junction , intracellular , biophysics , fluorescence recovery after photobleaching , fluorescence , confocal , extremely high frequency , chemistry , materials science , optics , optoelectronics , biology , physics , biochemistry , in vitro
Abstract The effect of 30.16 GHz millimeter wave (MMW) exposure at 1.0 and 3.5 mW/cm 2 on gap junction intercellular communication (GJIC) was studied in cultured HaCaT keratinocytes, using the fluorescence recovery after photobleaching (FRAP) technique and laser confocal scanning microscopy to follow the intracellular movement of 5,6‐carboxyfluorescein diacetate dye. While MMW exposure alone for 1 h at either 1.0 or 3.5 mW/cm 2 did not affect GJIC, MMW exposure in combination with 5 ng/ml TPA treatment reversed TPA induced suppression of GJIC. Exposure at 1.0 mW/cm 2 resulted in a partial reversal, and exposure at 3.5 mW/cm 2 resulted in essentially full reversal of the TPA suppression. Bioelectromagnetics 25:1–4, 2004. © 2003 Wiley‐Liss, Inc.