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Effects of ELF magnetic field on membrane protein structure of living HeLa cells studied by Fourier transform infrared spectroscopy
Author(s) -
Ikehara Toshitaka,
Yamaguchi Hisao,
Hosokawa Keiko,
Miyamoto Hiroshi,
Aizawa Katsuo
Publication year - 2003
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.10120
Subject(s) - absorbance , attenuated total reflection , infrared , infrared spectroscopy , protein secondary structure , spectroscopy , fourier transform infrared spectroscopy , analytical chemistry (journal) , hela , magnetic field , chemistry , nuclear magnetic resonance , membrane , materials science , optics , cell , biochemistry , physics , chromatography , organic chemistry , quantum mechanics
The effects of exposure to a 50 Hz magnetic field (maximum of 41.7 to 43.6 mT) on the membrane protein structures of living HeLa cells were studied using attenuated total reflection infrared spectroscopy. One min of such exposure shifted peak absorbance of the amide I band to a smaller wave number, reduced peak absorbance of the amide II band, and increased absorbance at around 1600 cm −1 . These results suggest that exposure to the ELF magnetic field has reversible effects on the N–H inplane bending and C–N stretching vibrations of peptide linkages, and changes the secondary structures of α‐helix and β‐sheet in cell membrane proteins. Bioelectromagnetics 24:457–464, 2003. © 2003 Wiley‐Liss, Inc.