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In vitro studies of the influence of ELF electromagnetic fields on the activity of soluble and insoluble peroxidase
Author(s) -
Portaccio M.,
De Luca P.,
Durante D.,
Rossi S.,
Bencivenga U.,
Canciglia P.,
Lepore M.,
Mattei A.,
De Maio A.,
Mita D.G.
Publication year - 2003
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.10119
Subject(s) - horseradish peroxidase , membrane , enzyme , peroxidase , in vivo , chemistry , in vitro , enzyme assay , bilayer , biochemistry , biophysics , covalent bond , biological membrane , microsome , organic chemistry , biology , microbiology and biotechnology
Abstract The influence of an extremely low frequency (ELF) magnetic field (50 Hz and 1 mT, EMF) on the activity of a soluble and insoluble horseradish peroxidase (E.C. 1.11.17) has been studied as a function of time. Insoluble derivatives were obtained by enzyme entrapment into two different gelatin membranes or by covalent attachment of the enzyme on two nylon membranes, differently preactivated. Results have shown that the field affects the inactivation rate of the soluble enzyme, while no effects are observed with insoluble derivatives. Since in vivo enzymes are immobilised into the biomembrane bilayer or entrapped into the cytoplasmic mixture, one might speculate that our experimental conditions do not reflect the catalytic activity of the enzymes in vivo. Bioelectromagnetics 24:449–456, 2003. © 2003 Wiley‐Liss, Inc.