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Elliptically polarized magnetic fields do not alter immediate early response genes expression levels in human glioblastoma cells
Author(s) -
Yomori Hiroyuki,
Yasunaga Katsuaki,
Takahashi Chie,
Tanaka Ayako,
Takashima Shinji,
Sekijima Masaru
Publication year - 2002
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.101
Subject(s) - gene expression , gene , bioelectromagnetics , glioblastoma , downregulation and upregulation , microbiology and biotechnology , elliptical polarization , biology , cancer research , physics , linear polarization , magnetic field , genetics , optics , quantum mechanics , laser
Expression of immediate early response genes such as c‐fos , c‐jun , and c‐myc in response to 1‐500 μT resultant (r) 60 Hz elliptically polarized (EP) magnetic fields (MFs), typical of environmental MFs polarization under overhead power lines, was analyzed in both at transcriptional and translational levels using human glioblastoma (T98G) cells. Pseudo synchronized T98G cells at G 1 phase were exposed to EP‐MFs (1, 20, 100, and 500 μTr) for up to 3 h, but produced no statistical difference ( P >0.05) in the levels of expression ratio at both the transcriptional and translational levels at 30 min for c‐fos and c‐jun and at 180 min for c‐myc after serum stimulation. In addition, exposure of T98G cells to linearly (vertical and horizontal) and/or circularly polarized MFs (500 μTr) produced no significant change ( P >0.05) in the expression ratio at both transcriptional and post‐transcriptional levels. Thus, there was no evidence that linearly or rotating polarized MFs enhanced early response gene expression in these studies. These results suggest that environmental MFs at 1–500 μT flux density are unlikely to induce carcinogenesis through a mechanism involving altered expression of the immediate early response genes. Bioelectromagnetics 23:89–96, 2002. © 2002 Wiley‐Liss, Inc.