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No effects of extremely low frequency magnetic fields found on cytotoxic activities and cytokine production of human peripheral blood mononuclear cells in vitro
Author(s) -
Ikeda Keiichi,
Shinmura Yasushi,
Mizoe Hiroki,
Yoshizawa Hiroaki,
Yoshida Atsuya,
Kanao Seiichi,
Sumitani Hiroyuki,
Hasebe Setsu,
Motomura Toshio,
Yamakawa Taku,
Mizuno Fumio,
Otaka Yoshihisa,
Hirose Hideki
Publication year - 2003
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.10062
Subject(s) - peripheral blood mononuclear cell , cytokine , tumor necrosis factor alpha , cytotoxic t cell , immunology , interferon gamma , lymphokine , immune system , biology , medicine , in vitro , biochemistry
Several epidemiologic studies have suggested an association between exposure to extremely low frequency (ELF) magnetic fields (MFs) and cancer in adults and children. A possible target of MFs is the immune system. The effects of the exposure to ELF MFs on the immunological functions of human peripheral blood mononuclear cells (PBMCs) obtained from healthy male volunteers were assessed by measuring the natural killer (NK) and lymphokine activated killer (LAK) activities and the production of interferon‐γ (IFN‐γ), tumor necrosis factor‐α (TNF‐α), interleukin‐2 (IL‐2), and interleukin‐10 (IL‐10). The PBMCs were exposed to three different MF: linearly polarized (vertical), circularly polarized, and elliptically polarized, at 50 and 60 Hz. Magnetic flux densities were set at 500, 100, 20, and 2 μT (rms) for vertical field and at 500 μT (rms) for the rotating fields. Using cytotoxicity assay and enzyme‐linked immunosorbent assay (ELISA) for cytokine production, we could not find any effects of ELF MFs on the cytotoxic activities and the cytokines production of human PBMCs. Bioelectromagnetics 24:21–31, 2003. © 2002 Wiley‐Liss, Inc.