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No effect of extremely low‐frequency magnetic field observed on cell growth or initial response of cell proliferation in human cancer cell lines
Author(s) -
Yoshizawa Hiroaki,
Tsuchiya Takehiro,
Mizoe Hiroki,
Ozeki Hidemi,
Kanao Seiichi,
Yomori Hiroyuki,
Sakane Chiharu,
Hasebe Setsu,
Motomura Toshio,
Yamakawa Taku,
Mizuno Fumio,
Hirose Hideki,
Otaka Yoshihisa
Publication year - 2002
Publication title -
bioelectromagnetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.435
H-Index - 81
eISSN - 1521-186X
pISSN - 0197-8462
DOI - 10.1002/bem.10006
Subject(s) - cell growth , cell culture , cell , magnetic field , cell division , cell cycle , extremely low frequency , dna synthesis , biology , chemistry , microbiology and biotechnology , nuclear magnetic resonance , physics , dna , biochemistry , genetics , quantum mechanics
An effect on the tumor promotion process, as represented by accelerated cell growth, has been indicated as one example of areas that demonstrate the possibility of biological effects of extremely‐low frequency magnetic fields. We, therefore, exposed the five cell lines (HL‐60, K‐562, MCF‐7, A‐375, and H4) derived from human tumors to a magnetic field for 3 days to investigate the effects on cell growth. Prior to exposure or sham exposure, the cells were precultured for 2 days in low serum conditions. The number of growing cells was counted in a blind manner. To investigate the effect on the initial response of cell proliferation, two cell lines were synchronized in G1 phase by serum starvation and then exposed to a magnetic field for 18 h (H4 cells) or 24 h (MCF‐7 cells), both with and without serum stimulation. The rate of DNA synthesis, taken as a measure of the cell proliferation, was determined by following the incorporation of [ 3 H]‐thymidine into the DNA. Three different magnetic field polarizations at both 50 and 60 Hz were used: linearly polarized (vertical); circularly polarized; and an elliptically polarized field. Magnetic field flux densities were set at 500, 100, 20 and 2 μT (rms) for the vertical field and at 500 μT (rms) for the rotating fields. No effect of magnetic field exposure was observed on either cell growth or the initial response of cell proliferation. Bioelectromagnetics 23:355–368, 2002. © 2002 Wiley‐Liss, Inc.