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Genetic deficiency of carnitine/organic cation transporter 2 ( slc22a5 ) is associated with altered tissue distribution of its substrate pyrilamine in mice
Author(s) -
Kato Sayaka,
Kato Yukio,
Nakamura Tadakatsu,
Sugiura Tomoko,
Kubo Yoshiyuki,
Deguchi Yoshiharu,
Tsuji Akira
Publication year - 2009
Publication title -
biopharmaceutics and drug disposition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 58
eISSN - 1099-081X
pISSN - 0142-2782
DOI - 10.1002/bdd.681
Subject(s) - pyrilamine , organic cation transport proteins , carnitine , pharmacology , medicine , endocrinology , kidney , in vivo , chemistry , transporter , biology , biochemistry , antagonist , receptor , microbiology and biotechnology , gene
Carnitine/organic cation transporter 2 (OCTN2) recognizes various cationic compounds as substrates in vitro , but information on its pharmacokinetic role in vivo is quite limited. This paper demonstrates altered tissue distribution of the OCTN2 substrate pyrilamine in juvenile visceral steatosis ( jvs ) mice, which have a hereditary defect of the octn2 gene. At 30 min after intravenous injection of pyrilamine, the tissue‐to‐plasma concentration ratio ( K p ) in the heart and pancreas was higher, whereas the K p in kidney and testis was lower in jvs mice compared with wild‐type mice. Pyrilamine transport studies in isolated heart slices confirmed higher accumulation, together with lower efflux, of pyrilamine in the heart of jvs mice. The higher accumulation in heart slices of jvs mice was abolished by lowering the temperature, by increasing the substrate concentration, and in the presence of other H 1 antagonists or another OCTN2 substrate, carnitine, suggesting that OCTN2 extrudes pyrilamine from heart tissue. On the other hand, the lower distribution to the kidney of jvs mice was probably due to down‐regulation of a basolateral transporter coupled with OCTN2, because, in jvs mice, (i) the K p of pyrilamine in kidney assessed immediately after intravenous injection (∼1 min) was also lower, (ii) the urinary excretion of pyrilamine was lower, and (iii) the uptake of pyrilamine in kidney slices was lower. The renal uptake of pyrilamine was saturable ( K m ∼236 µ M ) and was strongly inhibited by cyproheptadine, astemizole, ebastine and terfenadine. The present study thus indicates that genetic deficiency of octn2 alters pyrilamine disposition tissue‐dependently. Copyright © 2009 John Wiley & Sons, Ltd.