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A simplified approach for evaluation of hepatic drugoxidizing activity with a simultaneous determination of caffeine and trimethadione and their demethylated metabolites in rats with a selective cytochrome P‐450 inducer
Author(s) -
Tanaka Einosuke,
Ishikawa Akio,
Yamamoto Yuji,
Uchida Eiji,
Kobayashi Shinichi,
Yasuhara Hajime,
Misawa Shogo
Publication year - 1992
Publication title -
biopharmaceutics and drug disposition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 58
eISSN - 1099-081X
pISSN - 0142-2782
DOI - 10.1002/bdd.2510130405
Subject(s) - paraxanthine , chemistry , theobromine , metabolite , caffeine , theophylline , pharmacology , oral administration , glucuronidation , pharmacokinetics , metabolism , medicine , endocrinology , microsome , cytochrome p450 , biochemistry , enzyme , cyp1a2
We determined the blood concentrations of caffeine (CA) and its three primary dimethylxanthine metabolites: theobromine (TB), paraxanthine (PX), and theophylline (TP), and trimethadione (TMO) and its demethylated metabolite dimethadione (DMO) after the simultaneous administration of CA (10 mg kg −1 ) and TMO (4 mg kg −1 ) to rats pretreated with phenobarbital (PB: 40 and 80 mg kg −1 i.p. daily for 3 days) and 3‐methylcholanthrene (MC: 10 and 20 mg kg −1 i.p. daily for 2 days). After the oral administration of CA and TMO, the PB‐pretreated rats showed a significant increase in TMO metabolism, whereas the CA metabolism was greatly accelerated in rats pretreated with MC. In five pretreated groups, there were correlations which were determined 1 h after the administration of CA and TMO, between the plasma half‐life ( t 1/2 ) of CA and the TB/CA, PX/CA, and TP/CA ratios. The coefficients of correlation (r) ranged from −0·881 to −0·908, and the coefficients of correlation between the CL of CA and the TB/CA, PX/CA, and TP/CA ratios ranged from 0·959 to 0·989. There were high correlations between the t 1/2 of TMO and the DMO/TMO ratio at 1 h after administration with r = −0·966, and between the CL of TMO and the DMO/TMO ratio with r = 0·971. The above results suggest that one blood sampling after the simultaneous administration of CA and TMO enables prediction of the degree of each hepatic drug‐oxidizing activity because the P‐450 isozymes involved in metabolism of CA and TMO are different.