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Use of a microbial model for the determination of drug effects on cell metabolism and energetics: Study of citrulline‐malate
Author(s) -
Briand Joël,
Blehaut Henri,
Calvayrac Régis,
LavalMartin Danielle
Publication year - 1992
Publication title -
biopharmaceutics and drug disposition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 58
eISSN - 1099-081X
pISSN - 0142-2782
DOI - 10.1002/bdd.2510130102
Subject(s) - energy charge , metabolism , ammonium , aeration , biochemistry , citrulline , chemistry , euglena gracilis , nucleotide , biology , arginine , adenylate kinase , enzyme , amino acid , organic chemistry , chloroplast , gene
Abstract Euglena gracilis can be used as a microbial model to study the effect of drugs on lactate metabolism and gluconeogenetic synthesis. The cell growth and metabolism have been characterized in a 33 mM lactate medium, non‐supplemented or supplemented by dl ‐malate or by l ‐citrulline alone or by the compound formed by the stoichiometric combination of the two components: the citrulline‐malate (Stimol®). The malate of the complex accelerated the ammonium disappearance, while the citrulline facilitated the lactate consumption. A synergistic action of the complex, by comparison with the additive effects of the individual components, on most of the parameters studied was detected. A remarkable resistance to anoxia, and a quicker recovery under aeration of the cells supplemented with CM, were evident: after carbonation for 2 min the total nucleotides in the medium were increased by 44 per cent with an unchanged energy charge; and after a prolonged (20 min) anoxia followed by an aeration, the capacities of the cells to synthesize ATP in the presence of excesses of both ADP and phosphate were two‐fold higher in Stimol® treated cells than in control.