Premium
Endogenous plasma N ‐acetylcysteine and single dose oral bioavailability from two different formulations as determined by a new analytical method
Author(s) -
Gabard Bernard,
Mascher Hermann
Publication year - 1991
Publication title -
biopharmaceutics and drug disposition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 58
eISSN - 1099-081X
pISSN - 0142-2782
DOI - 10.1002/bdd.2510120504
Subject(s) - bioavailability , chemistry , acetylcysteine , oral administration , endogeny , pharmacology , high performance liquid chromatography , chromatography , crossover study , pharmacokinetics , bioequivalence , plasma levels , biochemistry , medicine , antioxidant , alternative medicine , pathology , placebo
A method to quantitate N ‐acetylcysteine in plasma using reductive cleavage with tributyl‐phosphine and post‐HPLC column derivatisation with o ‐phthalaldehyde is described. Using this method, endogenous average concentrations of 0·08 μM were measured in 10 volunteers participating in a crossover study to compare the bioavailability of two different formulations of N ‐acetylcysteine. The drug was detected in plasma for up to 12h after administration of a single oral dose (200 mg); the C max values were up to 20 times the endogenous levels. The sensitivity and selectivity of the method should thus enable the behaviour of N ‐acetylcysteine after oral administration to be properly described and bioavailability studies to be performed.