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Simultaneous determination of methenamine and formaldehyde in the urine of humans after methenamine administration
Author(s) -
Gollamudi Ramachander,
Meyer Marvin C.,
Straughn Arthur B.
Publication year - 1979
Publication title -
biopharmaceutics and drug disposition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 58
eISSN - 1099-081X
pISSN - 0142-2782
DOI - 10.1002/bdd.2510010105
Subject(s) - formaldehyde , chemistry , urine , chromatography , grocott's methenamine silver stain , hydrochloric acid , hexamethylenetetramine , chloride , biochemistry , medicine , inorganic chemistry , staining , pathology , organic chemistry
Methenamine (hexamethylenetetramine) and its hydrolysis product formaldehyde are determined in the presence of each other in urine by a spectrophotometric method. Formaldehyde is assayed by a colour reaction with tryptophan, sulphuric acid and ferric chloride after precipitating methenamine by three treatments with mercuric chloride. Methenamine is indirectly analysed by hydrolysis to formaldehyde with dilute hydrochloric acid. Formaldehyde levels as low as 5·0 μg ml −1 in the presence of methenamine concentrations as high as 2·5 mg ml −1 can be assayed. Of practical significance is the feature that urine may be stored up to 1 week for analysis, by appropriate dilution and freezing, without excessive loss of methenamine or formaldehyde. The method was applied to the determination of the bioavailability of methenamine hippurate in ten human volunteers.