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Improved RP‐HPLC determination of quinine in plasma and whole blood stored on filter paper
Author(s) -
Kolawole J.A.,
Mustapha A.
Publication year - 2000
Publication title -
biopharmaceutics and drug disposition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 58
eISSN - 1099-081X
pISSN - 0142-2782
DOI - 10.1002/bdd.244
Subject(s) - chromatography , whole blood , quinine , chemistry , high performance liquid chromatography , solid phase extraction , extraction (chemistry) , detection limit , cartridge , coefficient of variation , sample preparation , filter paper , blood plasma , quinidine , analyte , pharmacology , biochemistry , medicine , materials science , surgery , malaria , immunology , metallurgy
Analysis of quinine in plasma and whole blood samples dried on filter paper is described. Sample preparation involves liquid extraction of plasma and whole blood from the filter paper and subsequent solid‐phase extraction using C8 Bond Elut cartridges. A reverse‐phase liquid chromatography system with UV detection and fluorescence detection was used. The analytical characteristics of the method are reported, with a quantification limit of 0.1 µg mL −1 and within an assay coefficient of variation of 5.6–8.4% in plasma and 6.5–12% in whole blood. Representative chromatograms are shown as a function of time for samples from human subjects after ingestion of a single 400‐mg dose of quinine sulphate. Quinidine, dihydroquinine and metabolites are well separated from quinine with a resolution of above 1 (Rs>1). Copyright © 2000 John Wiley & Sons, Ltd.