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Exploring the compatibility mechanism of ShengDiHuang Decoction based on the in situ single‐pass intestinal perfusion model
Author(s) -
Yang Hui,
Hao Qi,
Cheng Jiangxue,
Wang Mei,
Zou Junbo,
Zhang Xiaofei,
Guo Dongyan
Publication year - 2020
Publication title -
biopharmaceutics and drug disposition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.419
H-Index - 58
eISSN - 1099-081X
pISSN - 0142-2782
DOI - 10.1002/bdd.2211
Subject(s) - catalpol , emodin , chemistry , aucubin , pharmacology , decoction , traditional medicine , limonin , herb , biochemistry , glycoside , stereochemistry , iridoid , medicine , medicinal herbs
Affecting the absorption of active ingredients in the intestine serves as one of the important compatibility mechanisms of traditional Chinese medicine. The aim of this study was to investigate the compatibility mechanism of ShengDiHuang Decoction (SDHD) by using the single‐pass intestinal perfusion in situ model. The major effective ingredients, catalpol, aucubin, acteoside, rehmannioside D, rehmannioside A, rhein, aloe emodin, emodin, chrysophanol, and physcion, were determined by HPLC. By analysing the effects of different concentrations, different pH, intestinal segments, protein inhibitors, and tight junction regulators on SDHD absorption, it was found that catalpol, aucubin, rehmannioside D, rehmannioside A, acteoside, rhein, and chrysophanol may undergo active transport, while aloe‐emodin and emodin may undergo passive transport. Catalpol, aucubin, and rehmannioside D may be substrates of BCRP and MRP2, while rehmannioside A and rhein may be substrates of BCRP, and acteoside and chrysophanol may be substrates of P‐gp, BCRP and MRP2. By comparing the P app values of the major effective ingredients between single herb and herb‐pairs, the compatibility of rehmannia and rheum could significantly promote the absorption of components in rehmannia. It is verified that rheum has a synergistic effect on the absorption of rehmannia in SDHD.