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A rationally identified marine GH1 β‐glucosidase has distinguishing functional features for simultaneous saccharification and fermentation
Author(s) -
Sousa Amanda S.,
Melo Ricardo R.,
Miyamoto Renan Y.,
Morais Mariana A. B.,
Andrade Liliane P.,
Milan Natália,
Avila Mayara C.,
Souza Cláudia M.,
Adão Regina C.,
Scarpassa Josiane A.,
Vieira Plínio S.,
Santos Leandro V.,
Ramos Carlos H. I.,
Murakami Mario T.,
Zanphorlin Letícia M.
Publication year - 2020
Publication title -
biofuels, bioproducts and biorefining
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.931
H-Index - 83
eISSN - 1932-1031
pISSN - 1932-104X
DOI - 10.1002/bbb.2136
Subject(s) - bagasse , fermentation , hydrolysis , biomass (ecology) , chemistry , raw material , enzyme , biochemistry , pichia pastoris , biochemical engineering , lignocellulosic biomass , pulp and paper industry , food science , microbiology and biotechnology , biology , organic chemistry , recombinant dna , gene , ecology , engineering
The classical route for second‐generation ethanol from lignocellulosic biomass is hampered by high process costs, fostering the development of alternative strategies such as simultaneous saccharification and fermentation (SSF). However, the lack of compatible enzyme cocktails poses a challenge. In this study, the enzyme Em Bgl from the marine bacterium Exiguobacterium marinum was rationally identified based on structural and phylogenetic analyses, known desirable properties of close orthologs, and the ecological niche of its organism source. Em Bgl is a multifunctional and glucose‐tolerant enzyme that efficiently hydrolyzes cello‐oligosaccharides due to a positive‐subsite region that can accommodate long cello‐oligosaccharides without imposing steric impediments. The efficacy of Em Bgl in an SSF process was demonstrated using pretreated sugarcane bagasse as feedstock, yielding 28 g L −1 of ethanol in 30 h. The distinguishing functional properties of Em Bgl and its successful utilization in an SSF process highlight its potential in biotechnological applications in which lignocellulose deconstruction is desirable under milder temperatures © 2020 Society of Chemical Industry and John Wiley & Sons, Ltd.