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Bioconversion of organosolv‐treated bamboo into biobutanol integrated with on‐site hydrolytic enzyme production
Author(s) -
Xu Ning,
He Aiyong,
Xu Jiaxing,
Xin Fengxue
Publication year - 2019
Publication title -
biofuels, bioproducts and biorefining
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.931
H-Index - 83
eISSN - 1932-1031
pISSN - 1932-104X
DOI - 10.1002/bbb.2065
Subject(s) - organosolv , chemistry , bioconversion , enzymatic hydrolysis , hydrolysate , hydrolysis , cellulase , bamboo , biofuel , lignin , pulp and paper industry , food science , fermentation , organic chemistry , microbiology and biotechnology , botany , biology , engineering
The aim of this study is to convert organosolv‐treated bamboo into biobutanol hydrolyzed by lignocellulosic enzyme mixtures produced on site through solid state fermentation (SSF). Higher xylanase and β ‐xylosidase activity in crude hydrolytic enzyme mixtures produced from untreated bamboo under SSF contributed to a higher saccharification yield (76.1%) than commercial ones (67.3%). The modified organosolv pretreatment under mild conditions, without acid catalysts at 35 °C, could also improve the sugar yield from bamboo efficiently by reducing 29.8% of lignin content. Finally, in situ extraction using biodiesel as the extractant could improve the final butanol titer to 28.4 g L −1 with a yield of 0.24 g g −1 from the organosolv treated bamboo enzymatic hydrolysate, thus demonstrating the first investigation of biobutanol production from bamboo. The results suggested that a crude cellulase complex produced under SSF and organosolv pretreatment could efficiently provide bamboo hydrolysate for biobutanol production without commercial cellulase usage. This has potential for large‐scale biobutanol production from bamboo wastes. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd