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Enhanced chondrogenic marker expression of human mesenchymal stem cells by interaction with both TGF‐β3 and hyaluronic acid
Author(s) -
Bhang Suk Ho,
Jeon JeongYi,
La WanGeun,
Seong Jun Yeup,
Hwang Jin Wook,
Ryu Seong Eon,
Kim ByungSoo
Publication year - 2011
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.39
Subject(s) - chondrogenesis , mesenchymal stem cell , aggrecan , hyaluronic acid , fibronectin , extracellular matrix , chemistry , microbiology and biotechnology , transforming growth factor , cartilage , sox9 , type ii collagen , cell culture , biology , biochemistry , anatomy , pathology , gene expression , medicine , articular cartilage , osteoarthritis , alternative medicine , genetics , gene
This study was designed to evaluate the additive effects of transforming growth factor‐beta3 (TGF‐β3) and hyaluronic acid (HA) on chondrogenic differentiation of human mesenchymal stem cells (hMSCs). The hMSCs were cultured on collagen type I‐, HA‐, or fibronectin‐coated cell culture dishes with or without TGF‐β3 added to the culture medium. Four weeks after cell culture, chondrogenic differentiation of hMSCs was determined by evaluating the expression of cartilage‐specific markers using real‐time polymerase chain reaction, immunocytochemistry, and Western blot analysis. hMSCs cultured on HA‐coated dishes with TGF‐β3 supplementation revealed a prominent increase in collagen type II, aggrecan, and Sox9. When hMSCs were cultured without TGF‐β3 supplementation, only hMSCs cultured on HA‐coated dishes showed prominent expression of the cartilage‐specific markers. This study shows that chondrogenic differentiation of hMSCs can be enhanced additively by interactions with both a specific cell‐adhesion matrix and a soluble growth factor.