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Transformation of chenodeoxycholic acid by thermophilic Geobacillus stearothermophilus
Author(s) -
Afzal Mohammad,
Oommen Sosamma,
AlAwadi Samira
Publication year - 2011
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.34
Subject(s) - thermophile , chemistry , geobacillus stearothermophilus , chenodeoxycholic acid , transformation (genetics) , dehydrogenation , organic chemistry , stereochemistry , biochemistry , catalysis , enzyme , bile acid , gene
We performed a series of experiments with Geobacillus stearothermophilus , a thermophile isolated from oil‐contaminated soil in the Kuwaiti desert. The organism has a good potential for the transformation of a broad spectrum of organic molecules such as steroids, amino acids, and aromatic hydrocarbons. In the present study, we tested its potential for the transformation of a bile component, chenodeoxycholic acid (CDCA). Five transformed products, namely, cholic acid, methylcholate, methylchenodeoxycholate, 3α‐hydroxy‐7‐oxo‐5β‐cholanic acid, and 7α‐hydroxy‐3‐oxo‐5β‐cholanic acid, were the major transformation products catalyzed by G. stearothermophilus . Under aerobic conditions, no evidence of side chain degradation, ring cleavage, or dehydrogenation was found among the metabolites of CDCA. CDCA transformation by a thermophile is reported for the first time.