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The effect of carbon source supplementation on the production of poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) by Cupriavidus necator
Author(s) -
Fereidouni Mohammad,
Younesi Habibollah,
Daneshi Ali,
Sharifzadeh Mazyar
Publication year - 2011
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.29
Subject(s) - cupriavidus necator , polyhydroxyalkanoates , fructose , poly 3 hydroxybutyrate , food science , chemistry , carbon fibers , yield (engineering) , carbon source , nitrogen , biochemistry , bacteria , organic chemistry , biology , materials science , genetics , composite number , metallurgy , composite material
The objective of the present study was to investigate the ability of Cupriavidus necator to produce poly‐(3‐hydroxybutyrate‐ co ‐3‐hydroxyvalerate) on various carbon sources in batch cultivation. These results show that C. necator produces poly‐3‐hydroxybutyrate from single carbon sources. The highest poly‐3‐hydroxybutyrate (P3HB) content was achieved at growth on fructose in the exponential growth phase. The maximum yield of the P3HV content was obtained when fructose was mixed with acetate. The highest content P3HB‐ co ‐3HV was also achieved by C. necator when we supplied C‐excess and N‐ and P‐normal conditions. These results indicate that C. necator accumulates high polyhydroxyalkanoates (PHA) content by depleting these elements in the culture medium. Nitrogen and phosphorus limitation has no significant effect on the PHA production, whereas C‐excess leads to an increase in PHA formation of up to 92% PHAs of cell dry weight after growth on 5 g/L acetate and 40 g/L fructose.