Premium
A genetic algorithm for the optimization of the thermoinduction protocol for high‐level production of recombinant human‐like collagen from Escherichia coli
Author(s) -
Chi Lei,
Fan DaiDi,
Ma XiaoXuan,
Mi Yu,
Luo YanE,
Zhu ChenHui,
Zhu XiaoLi,
Xue WenJiao
Publication year - 2011
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.25
Subject(s) - recombinant dna , escherichia coli , bioreactor , molar ratio , nitrogen , biology , chemistry , biochemistry , gene , botany , catalysis , organic chemistry
Abstract Production of recombinant human‐like collagen (RHLC) by thermoinduction of recombinant Escherichia coli BL 21 during high cell density cultivation was investigated in a 30 L bioreactor. The effects of induction temperature ( T ), pH, and carbon‐to‐nitrogen molar ratio of the nutrient medium (C/N) were examined. The optimal thermoinduction protocol for RHLC production was determined by using a model coupling genetic algorithm and artificial neural networks. The optimal operating conditions were as follows: maintenance of induction temperature at 42°C for 3 H and then at 39.4°C until the end, induction pH at 7.03, and C/N at 4.8 (mol/mol). The theoretical maximum concentration of RHLC was 12.5 g/L, whereas the experimental value was 12.1 g/L under the optimal induction conditions.