Premium
Sensitive identification of prostate‐specific antigen by iron oxide nanoparticle antibody conjugates on the gap‐finger electrode surface
Author(s) -
Zhang Wei,
Li Kai,
Guo Jingyang,
Ma Tao,
Wang Dongqing,
Shi Sumei,
Gopinath Subash C. B.,
Gu Deqiang
Publication year - 2021
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.2012
Subject(s) - prostate cancer , detection limit , antigen , prostate specific antigen , antibody , conjugate , conjugated system , electrode , nanoparticle , chemistry , prostate , cancer , chromatography , medicine , materials science , immunology , nanotechnology , polymer , mathematics , mathematical analysis , organic chemistry
Researches have proved that increasing level of prostate‐specific antigen (PSA) is an indicator for the progression of prostate cancer. The present study was focused to determine the PSA level by using anti‐PSA antibody conjugated iron oxide nanoparticles, as the probe immobilized on the gap‐fingered electrode sensing surface. The detection limit and sensitivity were found at the level of 1.9 pg/mL on the linear regression curve ( y = 1.6939 x − 0.5671; R ² = 0.9878). A dose‐dependent liner range was found from 1.9 until 60 pg/mL. Further, PSA was spiked in human serum and did not affect the interaction of PSA and its antibody. This method of detection quantifies the level of PSA, which helps to diagnose prostate cancer at its earlier stage.