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Rapid and label‐free electrochemical DNA biosensor based on a facile one‐step electrochemical synthesis of rGO–PPy–( L ‐Cys)–AuNPs nanocomposite for the HTLV‐1 oligonucleotide detection
Author(s) -
Fani Mona,
Rezayi Majid,
Pourianfar Hamid R.,
Meshkat Zahra,
Makvandi Manoocher,
Gholami Mehrdad,
Rezaee Seyed Abdolrahim
Publication year - 2021
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1973
Subject(s) - biosensor , differential pulse voltammetry , detection limit , nanocomposite , electrochemistry , polypyrrole , combinatorial chemistry , materials science , electrochemical gas sensor , electrode , nuclear chemistry , cyclic voltammetry , nanotechnology , chemistry , chromatography
Human T cell leukemia virus type 1 (HTLV‐1) as the first human retrovirus is currently a serious endemic health challenge. Despite the use of assorted molecular or serological assays for HTLV‐1 detection, there are several limitations due to the lack of a confirmatory test that may affect the accuracy of the results. Herein, a novel label‐free biosensor for the detection of HTLV‐1 Tax gene has been reported. An electrochemical facile ecofriendly synthesis method has been demonstrated based on a synthesis of nanocomposite of reduced graphene oxide, polypyrrole, and gold nanoparticles (rGO–PPy–( l ‐Cys)–AuNPs) deposited on the surface of screen‐printed carbon electrode. Electrochemical techniques were used to characterize and study the electrochemical behavior of the rGO–PPy–( l ‐Cys)–AuNPs, which exhibited a stable reference peak at 0.21 V associated with hybridization forms by applying the differential pulse voltammetry. The designed DNA biosensor presented a wide linear range from 0.1 fM to 100 µM and a low detection limit of 20 atto‐molar. The proposed biosensor presented in this study provides outstanding selectivity, sensitivity, repeatability, and reproducibility.