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Purification of cell‐derived Japanese encephalitis virus by dual‐mode chromatography
Author(s) -
Zhang Fuliang,
Luo Jun,
Teng Man,
Xing Guangxu,
Guo Junqing,
Zhang Yihua
Publication year - 2021
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1960
Subject(s) - japanese encephalitis , virus , chromatography , ultrafiltration (renal) , immunogenicity , infectivity , virology , chemistry , sindbis virus , differential centrifugation , gel permeation chromatography , sepharose , biology , antigen , encephalitis , biochemistry , enzyme , rna , immunology , gene , organic chemistry , polymer
Purification of the enveloped virus poses a challenge as one must retain viral infectivity to preserve immunogenicity. The traditional process of virus purification is time‐consuming, laborious and hard to scale up. Here, a rapid, simple and extensible laboratory program for the purification of Japanese encephalitis virus (JEV) was developed by using differential centrifugation, ultrafiltration, Sepharose 4 fast flow gel chromatography, and Capto TM Core 700 chromatography. The entire process recovered 61.64% of the original virus, and the purified virus particles maintained good activity and immunogenicity. The purification process described has potential application in large‐scale production of high‐purity JEV.