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Primary metabolites from overproducing microbial system using sustainable substrates
Author(s) -
Srivastava Rajesh K.,
Akhtar Nasim,
Verma Malkhey,
Imandi Sarat Babu
Publication year - 2020
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1927
Subject(s) - bioprocess , metabolic engineering , biochemical engineering , microbial metabolism , metabolite , bioprocess engineering , primary metabolite , metabolic pathway , substrate (aquarium) , flux (metallurgy) , biology , synthetic biology , industrial microbiology , biochemistry , computational biology , strain (injury) , microbiology and biotechnology , bacteria , chemistry , metabolism , fermentation , enzyme , engineering , genetics , ecology , organic chemistry , paleontology , anatomy
Primary (or secondary) metabolites are produced by animals, plants, or microbial cell systems either intracellularly or extracellularly. Production capabilities of microbial cell systems for many types of primary metabolites have been exploited at a commercial scale. But the high production cost of metabolites is a big challenge for most of the bioprocess industries and commercial production needs to be achieved. This issue can be solved to some extent by screening and developing the engineered microbial systems via reconstruction of the genome‐scale metabolic model. The predicted genetic modification is applied for an increased flux in biosynthesis pathways toward the desired product. Wherein the resulting microbial strain is capable of converting a large amount of carbon substrate to the expected product with minimum by‐product formation in the optimal operating conditions. Metabolic engineering efforts have also resulted in significant improvement of metabolite yields, depending on the nature of the products, microbial cell factory modification, and the types of substrate used. The objective of this review is to comprehend the state of art for the production of various primary metabolites by microbial strains system, focusing on the selection of efficient strain and genetic or pathway modifications, applied during strain engineering.