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Purification and properties of a laccase from the mushroom Agaricus sinodeliciosus
Author(s) -
Hao Jingzhe,
Zhang Weiwei,
Wang Hexiang,
Ziya Nur,
Luo Ying,
Jia Peisong,
Zhang Guoqing,
Ng Tzibun
Publication year - 2021
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1926
Subject(s) - laccase , abts , agaricus , chemistry , malachite green , chromatography , size exclusion chromatography , cellulose , molecular mass , mushroom , nuclear chemistry , organic chemistry , enzyme , dpph , antioxidant , food science , adsorption
Abstract A homogeneous monomeric laccase (ASL) from Agaricus sinodeliciosus , with a molecular mass of 65 kDa, was isolated using ion‐exchange chromatography (CM‐cellulose and Q‐Sepharose) and gel‐filtration chromatography (Superdex 75). This laccase exhibited maximum activity at 50 °C and pH 5.0. Hg 2+ and Cd 2+ significantly inhibited its activity. The laccase displayed a K m value of 0.9 mM toward 2,2′‐azinobis‐(3‐ethylbenzthiazoline‐6‐sulfonate) (ABTS). In addition to ABTS, ASL exhibited higher affinity toward o‐toluidine and benzidine than other substrates. ASL is able to decolorize malachite green and Eriochrome black T.