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The response of yeast vacuolar proteins: A novel rapid tool for Salmonella sp. screening
Author(s) -
Nguyen NgocTu,
Lee Jaewoong,
Woo Sung Min,
Kim YangHoon,
Min Jiho
Publication year - 2021
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1910
Subject(s) - vacuole , salmonella , yeast , recombinant dna , saccharomyces cerevisiae , biology , microbiology and biotechnology , confocal microscopy , enzyme , biochemistry , green fluorescent protein , bacteria , cytoplasm , gene , genetics
Abstract Human health is recently affected by several factors in which food contamination is one of the most dangerous elements that damage directly on our bodies. In this study, we provided a novel approach for the rapid detection of Salmonella sp. at the molecular level using the response of Saccharomyces cerevisiae ’s vacuoles. First, an augmentation of vacuoles intensity was observed by confocal microscopy after treating Salmonella strains with yeast cells. Second, the vacuolar enzymes were isolated and then analyzed by two‐dimensional electrophoresis for the screening of specific biomarkers. After that, various recombinant yeasts containing exclusive biomarkers were constructed by fusing these biomarkers with several fluorescent proteins. Finally, the recombinant strains showed the ability to detect Salmonella strains specifically by appropriate fluorescent signals from 20 CFU/mL after 15 Min of exposure.