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Metagenomic insights into bioaugmentation and biovalorization of oily industrial wastes by lipolytic oleaginous yeast Yarrowia lipolytica during successive batch fermentation
Author(s) -
Louhasakul Yasmi,
Cheirsilp Benjamas,
Treu Laura,
Kougias Panagiotis G.,
Angelidaki Irini
Publication year - 2020
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1878
Subject(s) - yarrowia , bioaugmentation , yeast , fermentation , industrial microbiology , biology , food science , candida tropicalis , bacteria , metagenomics , lipase , microorganism , microbiology and biotechnology , biochemistry , enzyme , genetics , gene
The lipolytic oleaginous yeast Yarrowia lipolytica was used in the bioaugmentation and biovalorization of oily industrial wastes during successive‐batch fermentation. Five cycles of nonsterile successive batch fermentation with 70% medium replacement achieved the highest oil removal of 68.1 ± 5.60% and produced biomass and lipid yields of 0.213 ± 0.07 g/g‐COD and 146.2 ± 46.5 mg/g‐COD, respectively. The cell‐bound lipase activity observed in the system was 170.74 ± 32 U/L. The auto‐flocculation efficiency of the biomass was >90% within 60 Min. The microbial community changes between Y. lipolytica and indigenous microorganisms were monitored by metagenomic next‐generation sequencing of internal transcribed spacer rDNA regions for yeasts and 16S rRNA gene for bacteria. Ylipolytica lipolytica was retained in the consortium together with other indigenous strains until the fifth cycle. Other minor oleaginous yeasts such as Kodamaea ohmeri and Candida tropicalis as well as polyhydroxyalkanoate‐accumulating bacteria were found and are likely to have participated in lipid production. This study has shown the robustness of Y. lipolytica in nonsterile successive batch fermentation and its use could contribute greatly to the practical valorization of industrial wastes for lipids and lipases.