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Production of polyhydroxyalkanoates by halotolerant bacteria with volatile fatty acids from food waste as carbon source
Author(s) -
Wang Pan,
Chen Xi Teng,
Qiu Yin Quan,
Liang Xiao Fei,
Cheng Meng Meng,
Wang Yong Jing,
Ren Lian Hai
Publication year - 2019
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1848
Subject(s) - polyhydroxyalkanoates , halotolerance , bacillus cereus , food science , fermentation , chemistry , effluent , bacteria , bioconversion , acidogenesis , biology , organic chemistry , waste management , anaerobic digestion , methane , genetics , engineering
In this study, a halotolerant strain was isolated from high salinity leachate and identified as Bacillus cereus NT‐3. It can produce a high concentration of polyhydroxyalkanoates (PHAs) with no significant changes when NaCl concentration is up to 50 g/L. FTIR and NMR spectra of PHAs synthesized by Bacillus cereus NT‐3 were similar to the standard or previous results. Effluent from acidogenic fermentation of food waste and pure volatile fatty acids (VFAs) mixture was used as carbon source to check the effect of non‐VFAs compounds of the effluent on PHAs production. The maximum PHAs production was 0.42 g/L for effluent fermentation, whereas it was 0.34 g/L for pure VFAs fermentation, indicating that bacteria could use actual effluent in a better way. Furthermore, a mathematical model was established for describing kinetic behavior of bacteria using different carbon sources. These results provided a promising approach for PHAs biosynthesis with a low‐cost carbon source.