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Electropermeabilization of metastatic chondrosarcoma cells from primary cell culture
Author(s) -
Michel Olga,
Błasiak Piotr,
Saczko Jolanta,
Kulbacka Julita,
DrągZalesińska Małgorzata,
Rzechonek Adam
Publication year - 2019
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1809
Subject(s) - electroporation , electrochemotherapy , chondrosarcoma , cisplatin , cancer research , cell culture , in vitro , cytotoxic t cell , camptothecin , biology , pharmacology , medicine , chemotherapy , pathology , biochemistry , genetics , gene
Primary cell cultures are challenging, but reliable model reflecting tumor response in vitro . The study was designed to examine if the increased electropermeabilization can overcame initial drug insensitivity in chondrosarcoma cells from lung metastasis. We established a primary cell culture and evaluated the cytotoxic impact of four drugs—cisplatin (CDDP), camptothecin, 2‐methoxyestradiol, and leucovorin calcium (LeuCa). After determination of parameters allowing for electropermeabilization, we performed electrochemotherapy in vitro with the least toxic drugs—CDDP and LeuCa. Although combining CDDP and leucovorin together increased their toxicity and supported apoptosis, application of pulsed electric fields (PEFs) brought no advantage for their efficacy. The study emphasizes the need for introduction of primary cell cultures into studies on pulse electric fields as model frequently less sensitive to PEF‐based treatments than continuous cell lines.