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Construction of a fusion anti‐caries DNA vaccine in transgenic tomato plants for PAcA gene and cholera toxin B subunit
Author(s) -
Bai Guohui,
Tian Yuan,
Wu Jiayuan,
Gu Yu,
Chen Zhu,
Zeng Fengjiao,
Liu Jianguo
Publication year - 2019
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1806
Subject(s) - streptococcus mutans , cholera toxin , biology , gene , fusion gene , microbiology and biotechnology , dna vaccination , protein subunit , plasmid , transgene , fusion protein , genome , virology , recombinant dna , bacteria , genetics
Chronic bacterial infections in the oral cavity influence the development of dental caries. Mutans streptococci are the major pathogenic cause of dental caries. The World Health Organization (WHO) ranks dental caries, cancer, and cardiovascular diseases as the three major global diseases that need urgent preventative and curative measures. However, substantial evidence suggests that traditional prevention and treatment strategies are inefficient in reducing the prevalence of dental caries. For protection against caries, it is important to develop effective vaccines that induce anticolonizing immunity against Streptococcus mutans infections. In the present investigation, we constructed a fusion anti‐caries DNA vaccine (PAcA‐ctxB) through fusing A region of cell surface protein PAc (PAcA) coding gene of mutans streptococci with cholera toxin B subunit coding gene (CTB). Afterward, the plasmids were integrated into tomato genomes through agrobacterium‐mediated plant transformation technology. The presence of transgenes in the tomato genome was confirmed by PCR, β‐glucuronidase gene (GUS), and western blot. The expression of genes was confirmed at transcription and protein level. Altogether, the results presented herein showed that transgenic tomatoes may provide a useful system for the production of human caries antigen.