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Deletion of arcA , iclR , and tdcC in Escherichia coli to improve l ‐threonine production
Author(s) -
Ding Zhixiang,
Fang Yu,
Zhu Lifei,
Wang Jianli,
Wang Xiaoyuan
Publication year - 2019
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1789
Subject(s) - escherichia coli , threonine , production (economics) , biology , microbiology and biotechnology , biochemistry , gene , enzyme , economics , serine , macroeconomics
l ‐Threonine is an important amino acid supplemented in food, medicine, or feed. Starting from glucose, l ‐threonine production in Escherichia coli involves the glycolysis, TCA cycle, and the l ‐threonine biosynthetic pathway. In this study, how the l ‐threonine production in an l ‐threonine producing E. coli TWF001 is controlled by the three regulators ArcA, Cra, and IclR, which control the expression of genes involved in the glycolysis and TCA cycle, has been investigated. Ten mutant strains were constructed from TWF001 by different combinations of deletion or overexpression of arcA , cra , iclR , and tdcC . l ‐Threonine production was increased in the mutants TWF015 (Δ arcA Δ cra ), TWF016 (Δ arcAPcra::Ptrc ), TWF017 (Δ arcA Δ iclR ), TWF018 (Δ arcA Δ iclR Δ tdcC ), and TWF019 (Δ arcA Δ cra Δ iclR Δ tdcC ). Among these mutant strains, the highest l ‐threonine production (26.0 g/L) was obtained in TWF018, which was a 109.7% increase compared with the control TWF001. In addition, TWF018 could consume glucose more efficiently than TWF001 and produce less acetate. The results suggest that deletion of arcA , iclR , and tdcC could efficiently increase l ‐threonine production in E. coli .