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A conductive cell‐imprinted substrate based on CNT–PDMS composite
Author(s) -
Kavand Hanie,
Rahaie Mahdi,
Koohsorkhi Javad,
Haghighipour Nooshin,
Bonakdar Shahin
Publication year - 2019
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1741
Subject(s) - polydimethylsiloxane , materials science , substrate (aquarium) , nanotechnology , composite number , electrical conductor , wafer , carbon nanotube , molding (decorative) , photoresist , adhesion , tissue engineering , adhesive , cell adhesion , composite material , biomedical engineering , layer (electronics) , medicine , oceanography , geology
Cell function regulation is influenced by continuous biochemical and biophysical signal exchange within the body. Substrates with nano/micro‐scaled topographies that mimic the physiological niche are widely applied for tissue engineering applications. As the cartilage niche is composed of several stimulating factors, a multifunctional substrate providing topographical features while having the capability of electrical stimulation is presented. Herein, we demonstrate a biocompatible and conductive chondrocyte cell‐imprinted substrate using polydimethylsiloxane (PDMS) and carbon nanotubes (CNTs) as conductive fillers. Unlike the conventional silicon wafers or structural photoresist masters used for molding, cell surface topographical replication is challenging as biological cells showed extremely sensitive to chemical solvent residues during molding. The composite showed no significant difference compared with PDMS with regard to cytotoxicity, whereas an enhanced cell adhesion was observed on the conductive composite's surface. Integration of nanomaterials into the cell seeding scaffolds can make tissue regeneration process more efficient.