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Fabrication of silver chloride nanoparticles using a plant serine protease in combination with photoactivation and investigation of their biological activities
Author(s) -
Siritapetawee Jaruwan,
Limphirat Wanwisa,
Nantapong Nawarat,
Songthamwat Dujdow
Publication year - 2018
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1638
Subject(s) - silver nanoparticle , nuclear chemistry , dpph , nanoparticle , surface plasmon resonance , chemistry , bacillus cereus , materials science , nanotechnology , organic chemistry , bacteria , antioxidant , biology , genetics
Recently, the development of "green" methods for fabrication of silver nanoparticles (Ag‐NPs) has been emphasized, in view of their environmental safety, feasibility, and low cost. In this study, a serine protease, EuP‐82 from Euphorbia cf. lactea latex, was used to fabricate silver chloride nanoparticles (AgCl‐NPs) in phosphate‐buffered saline (pH 7.2), under the influence of visible light. The fabricated nanoparticles had a maximal surface plasmon resonance absorption peak at 435 nm. The size of the AgCl‐NPs, estimated by scanning electron microscopy, was 57 ± 14.7 nm. Energy dispersive X‐ray spectroscopy, X‐ray absorption spectroscopy, and X‐ray diffraction analysis confirmed that the fabricated Ag‐NPs were of the AgCl type. The fabricated nanoparticles had antioxidant activity, scavenging DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) radicals with IC 50 of 204 ± 1.8 μg/mL. The fabricated AgCl‐NPs had broad‐spectrum in vitro antimicrobial activities, acting against the Gram‐positive bacteria Staphylococcus aureus , methicillin‐resistant S. aureus (MRSA), and Bacillus cereus , and the Gram‐negative bacteria Escherichia coli and Pseudomonas aeruginosa . AgCl‐NPs also showed antifungal activity against Candida albicans and C. tropicalis . In addition, AgCl‐NPs showed antiprotozoal activity against Giardia lamblia , with IC 50 202 ± 2.1 μg/mL. Based on the biological activities of the fabricated AgCl‐NPs, they have the potential for widespread application in medicine and industry.