Premium
Generation and selection of naïve Fab library for parasitic antigen: Anti‐ Bm SXP antibodies for lymphatic filariasis
Author(s) -
Omar Noorsharmimi,
Hamidon Nurul Hamizah,
Yunus Muhammad Hafiznur,
Noordin Rahmah,
Choong Yee Siew,
Lim Theam Soon
Publication year - 2017
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1591
Subject(s) - brugia malayi , monoclonal antibody , antibody , phage display , antigen , microbiology and biotechnology , lymphatic filariasis , recombinant dna , biology , hybridoma technology , peptide library , virology , filariasis , immunology , helminths , gene , genetics , peptide sequence
Phage display has been applied successfully as a tool for the generation of monoclonal antibodies (mAbs). Naive antibody libraries are unique as they are able to overcome several limitations associated with conventional mAb generation methods like the hybridoma technology. Here, we performed an in vitro selection and generation of Fab antibodies against Brugia malayi SXP protein ( Bm SXP), a recombinant antigen for the detection of lymphatic filariasis. We developed a naïve multi ethnic Fab antibody library with an estimated diversity of 2.99 × 10 9 . The antibody library was used to screen for mAbs against Bm SXP recombinant antigen. Soluble monoclonal Fab antibodies against Bm SXP were successfully isolated from the naïve library. The Fab antibodies obtained were expressed and analyzed to show its binding capability. The diversity obtained from a pool of donors from various ethnic groups allowed for a diverse antibody library to be generated. The mAbs obtained were also functional in soluble form, which makes it useful for further downstream applications. We believe that the Fab mAbs are valuable for further studies and could also contribute to improvements in the diagnosis of filariasis.