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Microbial production of mannitol by Lactobacillus brevis 3‐A5 from concentrated extract of Jerusalem artichoke tubers
Author(s) -
Cao Hailong,
Yue Min,
Liu Gang,
Du Yuguang,
Yin Heng
Publication year - 2017
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1590
Subject(s) - mannitol , jerusalem artichoke , fermentation , lactobacillus brevis , hydrolysate , food science , sugar , chemistry , reducing sugar , hydrolysis , biology , biochemistry , bacteria , lactic acid , lactobacillus plantarum , genetics
Abstract In the present study, the conversion of the extract of Jerusalem artichoke tubers for mannitol production by Lactobacillus brevis 3‐A5 was investigated. When the bacterium utilized enzymatic hydrolysates of Jerusalem artichoke extract as the main substrates in batch fermentation, the significant decrease in mannitol productivity was observed when the initial concentration of reducing sugar increased. Then, a strategy of continuous fed‐batch fermentation was adopted for improving mannitol production with enzymatic hydrolysates of Jerusalem artichoke extract as main substrates. Although the concentration of mannitol could reach 199.86 g/L at the end of the fermentation, the productivity for the overall process of the fermentation was only 1.67 g/L/H. To improve the mannitol productivity with both higher yield and concentration, the simultaneous enzymatic saccharification and fermentation (SSF) was studied. In SSF, the mannitol production reached 176.50 g/L in 28 H with a productivity of 6.30 g/L/H and a yield of 0.68 g/g total sugar. Our study provides a cost‐effective and eco‐friendly method for mannitol production from a cheap biomass.