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Production of high concentration of l ‐lactic acid from oil palm empty fruit bunch by thermophilic Bacillus coagulans JI12
Author(s) -
Juturu Veeresh,
Wu Jin Chuan
Publication year - 2017
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1567
Subject(s) - bacillus coagulans , hydrolysate , lactic acid , hemicellulose , fermentation , food science , hydrolysis , chemistry , thermophile , cellulose , cellulase , yeast , yeast extract , xylose , biochemistry , bacteria , biology , enzyme , genetics
Thermophilic Bacillus coagulans JI12 was used to ferment hemicellulose hydrolysate obtained by acid hydrolysis of oil palm empty fruit bunch at 50 °C and pH 6, producing 105.4 g/L of l ‐lactic acid with a productivity of 9.3 g/L/H by fed‐batch fermentation under unsterilized conditions. Simultaneous saccharification and fermentation (SSF) was performed at pH 5.5 and 50 °C to convert both hemicellulose hydrolysate and cellulose–lignin complex in the presence of Cellic Ctec2 cellulases using yeast extract (20 g/L) as the nitrogen source, giving 114.0 g/L of l ‐lactic acid with a productivity of 5.7 g/L/H. The SSF was also conducted by replacing yeast extract with equal amount of dry Bakers’ yeast, achieving 120.0 g/L of l ‐lactic acid with a productivity of 4.3 g/L/H. To the best of our knowledge, these lactic acid titers and productivities are the highest ever reported from lignocellulose hydrolysates.