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DNAzyme–aptamer or aptamer–DNAzyme paradigm: Biochemical approach for aflatoxin analysis
Author(s) -
Jafari Marzieh,
Rezaei Mohsen,
Kalantari Heibatullah,
Tabarzad Maryam,
Daraei Bahram
Publication year - 2017
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1563
Subject(s) - deoxyribozyme , aptamer , chemistry , biosensor , biochemistry , dna , circular dichroism , biophysics , biology , microbiology and biotechnology
DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to differentiate between these two conjugation patterns. Results showed that DNAzyme–aptamer has more catalytic activity and efficiency than aptamer–DNAzyme. Thereby, DNAzyme–aptamer with its superior efficiency can be used for design and development of more sensitive aflatoxin B1 DNA based biosensors.