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Gene expression profiling analysis of the effects of low‐intensity pulsed ultrasound on induced pluripotent stem cell–derived neural crest stem cells
Author(s) -
Xia Bin,
Zou Yang,
Xu Zhiling,
Lv Yonggang
Publication year - 2017
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1554
Subject(s) - induced pluripotent stem cell , neural crest , stem cell , microbiology and biotechnology , biology , neural stem cell , gene expression , gene expression profiling , gene , embryonic stem cell , genetics , embryo
Low‐intensity pulsed ultrasound (LIPUS) is a noninvasive technique that has been shown to affect cell proliferation, migration, and differentiation and promote the regeneration of damaged peripheral nerve. Our previous studies had proved that LIPUS can significantly promote the neural differentiation of induced pluripotent stem cell–derived neural crest stem cells (iPSCs‐NCSCs) and enhance the repair of rat‐transected sciatic nerve. To further explore the underlying mechanisms of LIPUS treatment of iPSCs‐NCSCs, this study reported the gene expression profiling analysis of iPSCs‐NCSCs before and after LIPUS treatment using the RNA‐sequencing (RNA‐Seq) method. It was found that expression of 76 genes of iPSCs‐NCSCs cultured in a serum‐free neural induction medium and expression of 21 genes of iPSCs‐NCSCs cultured in a neuronal differentiation medium were significantly changed by LIPUS treatment. The differentially expressed genes are related to angiogenesis, nervous system activity and functions, cell activities, and so on. The RNA‐seq results were further verified by a quantitative real‐time reverse transcriptase polymerase chain reaction (qRT‐PCR). High correlation was observed between the results obtained from qRT‐PCR and RNA‐Seq. This study presented new information on the global gene expression patterns of iPSCs‐NCSCs after LIPUS treatment and may expand the understanding of the complex molecular mechanism of LIPUS treatment of iPSCs‐NCSCs.

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