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Large‐scale purification and characterization of recombinant human stem cell factor in Escherichia coli
Author(s) -
Chen Lianghua,
Cai Feng,
Zhang Danju,
Zhang Li,
Zhu Peng,
Gao Shun
Publication year - 2017
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1517
Subject(s) - escherichia coli , recombinant dna , inclusion bodies , chromatography , denaturation (fissile materials) , chemistry , sepharose , affinity chromatography , specific activity , biochemistry , biology , enzyme , gene , nuclear chemistry
The pharmacological importance of recombinant human stem cell factor (rhSCF) has increased the demand to establish effective and large‐scale production and purification processes. A good source of bioactive recombinant protein with capability of being scaled‐up without losing activity has always been a challenge. The objectives of the study were the rapid and efficient pilot‐scale expression and purification of rhSCF. The gene encoding stem cell factor (SCF) was cloned into pBV220 and transformed into Escherichia coli . The recombinant SCF was expressed and isolated using a procedure consisting of isolation of inclusion bodies (IBs), denaturation, and refolding followed by chromatographic steps toward purification. The yield of rhSCF reached 835.6 g/20 L, and the expression levels of rhSCF were about 33.9% of the total E. coli protein content. rhSCF was purified by isolation of IBs, denaturation, and refolding, followed by SP‐Sepharose chromatography, Source 30 reversed‐phase chromatography, and Q‐Sepharose chromatography. This procedure was developed to isolate 5.5 g of rhSCF (99.5% purity) with specific activity at 0.96 × 10 6 IU/mg, endotoxin levels of pyrogen at 1.0 EU/mg, and bacterial DNA at 10 ng/mg. Pilot‐scale fermentations and purifications were set up for the production of rhSCF that can be upscaled for industry.