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Unusual poly(3‐hydroxyalkanoate) (PHA) biosynthesis behavior of Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002 isolated from palm oil mill effluent
Author(s) -
RazaifMazinah Mohd Rafais Mohd,
Anis Siti Nor Syairah,
Harun Hazwani Izzati,
Rashid Khairunnisa Abdul,
Annuar Mohamad Suffian Mohamad
Publication year - 2016
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1482
Subject(s) - pseudomonas putida , polyhydroxyalkanoates , glycerol , chemistry , lauric acid , myristic acid , sugar , bacteria , food science , fatty acid , organic chemistry , chromatography , nuclear chemistry , palmitic acid , biology , enzyme , genetics
Pseudomonas putida Bet001 and Delftia tsuruhatensis Bet002, isolated from palm oil mill effluent, accumulated poly(3‐hydroxyalkanoates) (PHAs) when grown on aliphatic fatty acids, sugars, and glycerol. The substrates were supplied at 20:1 C/N mole ratio. Among C‐even n ‐alkanoic acids, myristic acid gave the highest PHA content 26 and 28 wt% in P. putida and D. tsuruhatensis , respectively. Among C‐odd n ‐alkanoic acids, undecanoic gave the highest PHA content at 40 wt% in P. putida and 46 wt% in D. tsuruhatensis on pentadecanoic acid. Sugar and glycerol gave <10 wt% of PHA content for both bacteria. Interestingly, D. tsuruhatensis accumulated both short‐ and medium‐chain length PHA when supplied with n ‐alkanoic acids ranging from octanoic to lauric, sucrose, and glycerol with 3‐hydroxybutyrate as the major monomer unit. In P. putida , the major hydroxyalkanoates unit was 3‐hydroxyoctanoate and 3‐hydroxydecanoate when grown on C‐even acids. Conversely, 3‐hydroxyheptanoate, 3‐hydrxoynonanoate, and 3‐hydroxyundecanoate were accumulated with C‐odd acids. Weight‐averaged molecular weight ( M w ) was in the range of 53–81 kDa and 107–415 kDa for P. putida and D. tsuruhatensis , respectively. Calorimetric analyses indicated that both bacteria synthesized semicrystalline polymer with good thermal stability with degradation temperature ( T d ) ranging from 178 to 282 °C.