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Butanol production by a Clostridium beijerinckii mutant with high ferulic acid tolerance
Author(s) -
Liu Jun,
Guo Ting,
Wang Dong,
Xu Jiahui,
Ying Hanjie
Publication year - 2015
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1418
Subject(s) - clostridium beijerinckii , ferulic acid , butanol , chemistry , hydrolysate , biochemistry , food science , fermentation , ethanol , hydrolysis
Abstract A mutant strain of Clostridium beijerinckii , with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high‐throughput screening of C. beijerinckii NCIMB 8052. The mutant strain M11 produced 7.24 g/L of butanol when grown in P2 medium containing 30 g/L of glucose and 0.5 g/L of ferulic acid, which is comparable to the production from non‐ferulic acid cultures (8.11 g/L of butanol). When 0.8 g/L of ferulic acid was introduced into the P2 medium, C. beijerinckii M11 grew well and produced 4.91 g/L of butanol. Both cell growth and butanol production of C. beijerinckii M11 were seriously inhibited when 0.9 g/L of ferulic acid was added into the P2 medium. Furthermore, C. beijerinckii M11 could produce 6.13 g/L of butanol using non‐detoxified hemicellulosic hydrolysate from diluted sulfuric acid‐treated corn fiber (SAHHC) as the carbon source. These results demonstrate that C. beijerinckii M11 has a high ferulic acid tolerance and is able to use non‐detoxified SAHHC for butanol production.