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Graphene oxide–mediated electrochemistry of glucose oxidase on glassy carbon electrodes
Author(s) -
Castrignanò Silvia,
Valetti Francesca,
Gilardi Gianfranco,
Sadeghi Sheila J.
Publication year - 2015
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1392
Subject(s) - glucose oxidase , glassy carbon , graphene , biosensor , lyotropic , amperometry , electrochemistry , inorganic chemistry , electrode , chemistry , oxide , biomolecule , chemical engineering , materials science , cyclic voltammetry , nanotechnology , organic chemistry , polymer , liquid crystalline , engineering
Glucose oxidase (GOD) was immobilized on glassy carbon electrodes in the presence of graphene oxide (GO) as a model system for the interaction between GO and biological molecules. Lyotropic properties of didodecyldimethylammonium bromide (DDAB) were used to stabilize the enzymatic layer on the electrode surface resulting in a markedly improved electrochemical response of the immobilized GOD. Transmission electron microscopy images of the GO with DDAB confirmed the distribution of the GO in a two‐dimensional manner as a foil‐like material. Although it is known that glassy carbon surfaces are not ideal for hydrogen peroxide detection, successful chronoamperometric titrations of the GOD in the presence of GO with β‐ d ‐glucose were performed on glassy carbon electrodes, whereas no current response was detected upon β‐ d ‐glucose addition in the absence of GO. The GOD–DDAB–GO system displayed a high turnover efficiency and substrate affinity as a glucose biosensor. The simplicity and ease of the electrode preparation procedure of this GO/DDAB system make it a good candidate for immobilizing other biomolecules for fabrication of amperometric biosensors.