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Effect of several PEO–PPO amphiphiles on bax , bcl‐2 , and hTERT mRNAs: An insight into apoptosis and cell immortalization induced in hepatoma cells by these polymeric excipients
Author(s) -
Castronuovo Cynthia Celeste,
Cuestas María Luján,
Oubiña José Raúl,
Mathet Verónica Lidia
Publication year - 2015
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1352
Subject(s) - apoptosis , telomerase reverse transcriptase , flow cytometry , microbiology and biotechnology , telomerase , cell growth , cell culture , chemistry , cell , downregulation and upregulation , biology , biochemistry , gene , genetics
Recent data have shown that synthetic polymers and nanomaterials display phenotypic effects in cells and signal transduction mechanisms involved in inflammation, differentiation, proliferation, and apoptosis. Aim: This article aims to investigate the effect of poly(ethylene oxide)–poly(propylene oxide) (PEO–PPO) block copolymers with a wide range of biomedical and pharmaceutical applications on apoptosis and/or cell immortalization, by flow cytometry and multiplex RT‐PCR for bax , bcl‐ 2, and human telomerase reverse transcriptase ( hTERT) . Results: PEO–PPO amphiphiles upregulated bax and hTERT and induced apoptosis of two human hepatoma cell lines. Conclusions: PEO–PPO block copolymers—considered safe for human use—can drastically alter gene expression profiles of genes related to apoptosis/cell proliferation.