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Screening and characterization of a thermostable lipase from marine Streptomyces sp. strain W007
Author(s) -
Yuan Dongjuan,
Lan Dongming,
Xin Ruipu,
Yang Bo,
Wang Yonghua
Publication year - 2015
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1338
Subject(s) - lipase , triglyceride lipase , bacillus subtilis , triacylglycerol lipase , monoacylglycerol lipase , biochemistry , hydrolysis , enzyme , pichia pastoris , streptomyces , biology , chemistry , bacteria , recombinant dna , endocannabinoid system , genetics , receptor , gene
Abstract A screening method along with the combination of genome sequence of microorganism, pairwise alignment, and lipase classification was used to search the thermostable lipase. Then, a potential thermostable lipase (named MAS1) from marine Streptomyces sp. strain W007 was expressed in Pichia pastoris X‐33, and the biochemical properties were characterized. Lipase MAS1 belongs to the subfamily I.7, and it has 38% identity to the well‐characterized Bacillus subtilis thermostable lipases in the subfamily I.4. The purified enzyme was estimated to be 29 kDa. The enzyme showed optimal temperature at 40 °C, and retained more than 80% of initial activity after 1 H incubation at 60 °C, suggesting that MAS1 was a thermostable lipase. MAS1 was an alkaline enzyme with optimal pH value at 7.0 and had stable activity for 12 H of incubation at pH 6.0–9.0. It was stable and retained about 90% of initial activity in the presence of Cu 2+ , Ca 2+ , Ni 2+ , and Mg 2+ , whereas 89.05% of the initial activity was retained when ethylene diamine tetraacetic acid was added. MAS1 showed the tolerance to organic solvents, but was inhibited by various surfactants. MAS1 was verified to be a triglyceride lipase and could hydrolyze triacylglycerol and diacylglycerol. The result represents a good example for researchers to discover thermostable lipase for industrial application.