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Biofunctionalized silver nanoparticles as a novel colorimetric probe for melamine detection in raw milk
Author(s) -
Borase Hemant P.,
Patil Chandrashekhar D.,
Salunkhe Rahul B.,
Suryawanshi Rahul K.,
Salunke Bipinchandra K.,
Patil Satish V.
Publication year - 2015
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1306
Subject(s) - melamine , silver nanoparticle , detection limit , surface plasmon resonance , chemistry , nanoparticle , transmission electron microscopy , nuclear chemistry , analytical chemistry (journal) , nanotechnology , chromatography , materials science , organic chemistry
Nanoparticles have emerged as a promising analytical tool for monitoring food adulteration and safety. In the present study, silver nanoparticles (AgNPs) were synthesized using leaves' extract of Jatropha gossypifolia . AgNPs revealed a characteristic surface plasmon resonance (SPR) peak at 419 nm and have spherical and grain shape with size range between 18 and 30 nm. A selective and rapid method of melamine detection in raw milk was developed with the use of these biofunctionalized AgNPs. The color change, deviation in SPR spectra, and change in the absorption ratio ( A 500 / A 419 ) of AgNPs occurred after an AgNPs–melamine interaction. The detection limit for melamine up to 2 μM (252 ppb) was attained with this method, which is quite lower than safety level recommendations of regulatory bodies demonstrating sensitivity of the method. Dynamicx light scattering and transmission electron microscopy analyses exhibited an increase in hydrodynamic diameter and size of AgNPs after melamine interaction. Melamine sensing by AgNPs was investigated by different physicochemical and thermal analyses.