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Research on protein thermal condensation detection based on phase modulation SPR imaging
Author(s) -
He Peng,
Kang ZiYang,
Wan XiaoQing,
Fang HanXiong
Publication year - 2016
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1300
Subject(s) - lysozyme , condensation , interference (communication) , modulation (music) , phase (matter) , biological system , protein array analysis , phase modulation , chemistry , nanotechnology , materials science , computer science , physics , biology , dna microarray , acoustics , channel (broadcasting) , computer network , biochemistry , gene expression , organic chemistry , gene , thermodynamics
This article presents a novel SPR imaging biomolecular interaction detection method based on time domain phase modulation. An experimental apparatus of SPR imaging biomolecular interaction detection based on TDPM is established to detect biomolecular interaction. During the experimental pretreatment process, we prepared the 2×2 lysozyme array chip and detected lysozyme thermal condensation state with the experimental apparatus. Using the Stoilov algorithm, we were able to extract the changed phase information as well as obtain the interactive SPR curves and calculate kinetic parameters. This method can sensitively acquire real‐time phase change caused by biomolecular interaction based on interference imaging and resolve the related bioinformation, which is a potential tool for proteomics research.