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Extraction of antibiotic zwittermicin A from Bacillus thuringiensis by macroporous resin and silica gel column chromatography
Author(s) -
Hao Zaibin,
Yan Li,
Liu Jianguo,
Song Fuping,
Zhang Jie,
Li Xia
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1277
Subject(s) - bacillus thuringiensis , chromatography , bacillus subtilis , ninhydrin , silica gel , column chromatography , chemistry , extraction (chemistry) , antibacterial activity , staphylococcus aureus , yield (engineering) , bacteria , biology , biochemistry , materials science , genetics , amino acid , metallurgy
To establish a production process capable of providing refined zwittermicin A (ZwA) on a large scale, the macroporous resin and silica gel column chromatography were used to separate and purify the antibiotic ZwA from the fermentation broth of Bacillus thuringiensis HD‐1. The result of high‐performance liquid chromatography–mass spectrometry after purification suggests that the samples of ZwA were of high purity, 89%, and the average yield was 20 mg L −1 . Erwinia herbicola LS005, Escherichia coli , Staphylococcus aureus , and Bacillus subtilis were used to assess the toxicity of ZwA. The antibiotic had strong antibacterial activity against E. herbicola LS005 and a color reaction with ninhydrin.