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Nutrient media optimization for simultaneous enhancement of the laccase and peroxidases production by coculture of D ichomitus squalens and C eriporiopsis subvermispora
Author(s) -
Kannaiyan Ranjani,
Mahinpey Nader,
Kostenko Victoria,
Martinuzzi Robert J.
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1263
Subject(s) - laccase , chemistry , manganese peroxidase , peroxidase , sodium nitrate , casein , food science , arabinose , lignin , biochemistry , enzyme , fermentation , xylose , organic chemistry
Coculturing of two white‐rot fungi, D ichomitus squalens and C eriporiopsis subvermispora , was explored for the optimization of cultivation media for simultaneous augmentation of laccase and peroxidase activities by response surface methodology ( RSM ). Nutrient parameters chosen from our previous studies with the monocultures of D . squalens and C . subvermispora were used to design the experiments for the cocultivation study. Glucose, arabinose, sodium nitrate, casein, copper sulfate (CuSO 4 ), and manganese sulfate (MnSO 4 ) were combined according to central composite design and used as the incubation medium for the cocultivation. The interaction of glucose and sodium nitrate resulted in laccase and peroxidase activities of approximately 800 U/g protein. The addition of either glucose or sodium nitrate to the medium also modifies the impact of other nutrients on the ligninolytic activity. Both enzyme activities were cross‐regulated by arabinose, casein, CuSO 4 , and MnSO 4 as a function of concentrations. Based on RSM , the optimum nutrient levels are 1% glucose, 0.1% arabinose, 20 mM sodium nitrate, 0.27% casein, 0.31 mM CuSO 4 , and 0.07 mM MnSO 4 . Cocultivation resulted in the production of laccase of 1,378 U/g protein and peroxidase of 1,372 U/g protein. Lignin (16.9%) in wheat straw was degraded by the optimized enzyme mixture.