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Xylanolytic enzyme systems in Arthrobacter sp. MTCC 5214 and Lactobacillus sp.
Author(s) -
Khandeparker Rakhee,
Jalal Thasneem
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1253
Subject(s) - xylanase , xylan , arthrobacter , bacteria , marine bacteriophage , food science , chemistry , enzyme , bran , biochemistry , microorganism , xylose , carbon source , pseudomonas , biology , microbiology and biotechnology , fermentation , organic chemistry , raw material , genetics
The production of extracellular xylanolytic enzymes such as xylanase, α‐ l ‐arabinofuranosidase (α‐ l ‐AFase), and acetyl xylan esterase (Axe) by marine Arthrobacter sp. and Lactobacillus sp. was investigated using different carbon sources. Induction and repression of all these enzymes differed with carbon source and also with the organism. Wheat bran was the best carbon source for the production of xylanase and α‐ l ‐AFase, whereas both isolates showed maximum Axe production when grown on oat bran as a carbon source. Preferential utilization of a carbon source for enzyme production can give us better insights into regulatory mechanism in these marine bacteria. Elution profile as well as zymogram analysis indicated the possibility of bifunctional α‐ l ‐AFase–Axes in both marine bacteria.