Premium
Development of a paper‐type tyrosinase biosensor for detection of phenolic compounds
Author(s) -
Şenyurt Özge,
Eyidoğan Füsun,
Yılmaz Remziye,
Öz M. Tufan,
Özalp V. Cengiz,
Arıca Yakup,
Öktem Hüseyin A.
Publication year - 2014
Publication title -
biotechnology and applied biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.468
H-Index - 70
eISSN - 1470-8744
pISSN - 0885-4513
DOI - 10.1002/bab.1246
Subject(s) - biosensor , tyrosinase , catechol , hydrazone , chemistry , chromatography , substrate (aquarium) , chromophore , phenols , combinatorial chemistry , organic chemistry , enzyme , biochemistry , biology , ecology
A low‐cost, portable, and disposable paper‐type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper‐strip absorption method. Tyrosinase and a chromophore (3‐methyl‐2‐benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate ( l ‐dopamine). The biosensor was responsive to phenolic compounds such as 4‐chlorophenol, catechol, m ‐cresol, and p ‐cresol. The sensor showed stability for 70 days. The developed biosensor can be used for remote on‐site qualitative monitoring of phenolic compounds in wastewater samples.